pyrosequencing
Pyrosequencing(R) is a unique system that allows the analysis of genetic variations including single-nucleotide polymorphisms, indels and short repeats, as well as assessing RNA allelic imbalance, DNA methylation status, and gene copy number.
Advances in methodology, including multiplex and universal primer applications, have reduced assay cost and improved throughput.
Biotechs
References
Marsh S. Pyrosequencing(R) applications. Methods Mol Biol. 2006;373:15-24. PMID: 17185754
Examples
Spittle C, Ward MR, Nathanson KL, Gimotty PA, Rappaport E, Brose MS, Medina A, Letrero R, Herlyn M, Edwards RH. Application of a BRAF pyrosequencing assay for mutation detection and copy number analysis in malignant melanoma. J Mol Diagn. 2007 Sep;9(4):464-71. PMID: 17690212