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pyrosequencing
Sunday 4 February 2007
Pyrosequencing(R) is a unique system that allows the analysis of genetic variations including single-nucleotide polymorphisms, indels and short repeats, as well as assessing RNA allelic imbalance, DNA methylation status, and gene copy number.
Advances in methodology, including multiplex and universal primer applications, have reduced assay cost and improved throughput.
Biotechs
References
Pyrosequencing of IDH1 and IDH2 Mutations in Brain Tumors and Non-neoplastic Conditions. Cykowski MD, Allen RA, Fung KM, Harmon MA, Dunn ST. Diagn Mol Pathol. 2012 Dec;21(4):214-20. PMID: 23111198
Sensitive determination of BRAF copy number in clinical samples by pyrosequencing. Setty P, Gessi M, Waha A, Hammes J, El-Maarri O, Pietsch T, Waha A. Diagn Mol Pathol. 2011 Sep;20(3):148-57. PMID: 21817898
Marsh S. Pyrosequencing(R) applications. Methods Mol Biol. 2006;373:15-24. PMID: 17185754
Spittle C, Ward MR, Nathanson KL, Gimotty PA, Rappaport E, Brose MS, Medina A, Letrero R, Herlyn M, Edwards RH. Application of a BRAF pyrosequencing assay for mutation detection and copy number analysis in malignant melanoma. J Mol Diagn. 2007 Sep;9(4):464-71. PMID: 17690212
