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PAM-1


- The fully human germline-coded monoclonal IgM antibody PAM-1 was isolated from a patient with a stomach carcinoma.

- PAM-1 reacts with a post-transcriptionally modified isoform of membrane receptor CFR-1 which is overexpressed on almost all epithelial cancers of all types and origins.

- The CFR-1/PAM-1 receptor was expressed on nearly all precancerous stages and carcinomas while normal breast and prostate tissue showed negative results. These results were confirmed by FACS analysis showing a CFR-1/PAM-1 expression only on prostate carcinoma cells but not on benign prostate hyperplasia cells.

- The unique expression of this new CFR-1/PAM-1 receptor makes the PAM-1 antibody an ideal diagnostic and even therapeutic tool for precancerous and cancerous epithelial lesions of the breast and the prostate.

- This CFR-1/PAM-1 receptor is post-transcriptionally modified and over-expressed on human epithelial tumors and carcinoma pre-cancer lesions such as H. pylori induced gastritis, intestinal metaplasia and dysplasia of the stomach, ulcerative colitis-related dysplasia and adenomas of the colon, Barrett metaplasia and dysplasia of the esophagus, squamous cell metaplasia and dysplasia of the lung and cervical intraepithelial neoplasia.

- The expression of CFR-1/PAM-1 correlates with the proliferation rate and increases with the grade of malignancy.

- The human monoclonal antibody PAM-1 inhibits cell growth and induces apoptosis, in vitro and in vivo.

References

- Brandlein S, Eck M, Strobel P, Wozniak E, Muller-Hermelink HK, Hensel F, Vollmers HP. PAM-1, a natural human IgM antibody as new tool for detection of breast and prostate precursors. Hum Antibodies. 2004;13(4):97-104. PMID: 15719499

- Brandlein S, Pohle T, Vollmers C, Wozniak E, Ruoff N, Muller-Hermelink HK, Vollmers HP. CFR-1 receptor as target for tumor-specific apoptosis induced by the natural human monoclonal antibody PAM-1. Oncol Rep. 2004 Apr;11(4):777-84. PMID: 15010872

- Brandlein S, Beyer I, Eck M, Bernhardt W, Hensel F, Muller-Hermelink HK, Vollmers HP. Cysteine-rich fibroblast growth factor receptor 1, a new marker for precancerous epithelial lesions defined by the human monoclonal antibody PAM-1. Cancer Res. 2003 May 1;63(9):2052-61. PMID: 12727819