SILAC
stable-isotope labelling by amino acids in cell culture
Among the many formats for quantitative proteomics, stable-isotope labelling by amino acids in cell culture (SILAC) has emerged as a simple and powerful one.
SILAC removes false positives in protein-interaction studies, reveals large-scale kinetics of proteomes and - as a quantitative phosphoproteomics technology - directly uncovers important points in the signalling pathways that control cellular decisions.
References
Mann M. Functional and quantitative proteomics using SILAC. Nat Rev Mol Cell Biol. 2006 Dec;7(12):952-8. PMID: 17139335