NUT midline carcinoma (NMC) is a uniformly lethal malignancy that is defined by rearrangement of the nuclear protein in testis (NUT) gene on chromosome 15q14.
NMCs are morphologically indistinguishable from other poorly differentiated carcinomas, and the diagnosis is usually made currently by fluorescence in situ hybridization (FISH).
As normal NUT expression is confined to testis and ovary, we reasoned that an immunohistochemical (IHC) stain for NUT would be useful in diagnosing NMC.
IHC staining with the C52 monoclonal antibody is a highly sensitive and specific test that reliably distinguishes NMC from other forms of carcinoma. (19363441)
References
Diagnosis of NUT Midline Carcinoma Using a NUT-specific Monoclonal Antibody. Haack H, Johnson LA, Fry CJ, Crosby K, Polakiewicz RD, Stelow EB, Hong SM, Schwartz BE, Cameron MJ, Rubin MA, Chang MC, Aster JC, French CA. Am J Surg Pathol. 2009 Apr 9. PMID: 19363441
Molecular pathology of NUT midline carcinomas. French CA. J Clin Pathol. 2008 Jun 13. PMID: 18552174
BRD-NUT oncoproteins: a family of closely related nuclear proteins that block epithelial differentiation and maintain the growth of carcinoma cells. French CA, Ramirez CL, Kolmakova J, Hickman TT, Cameron MJ, Thyne ME, Kutok JL, Toretsky JA, Tadavarthy AK, Kees UR, Fletcher JA, Aster JC. Oncogene. 2008 Apr 3;27(15):2237-42. PMID: 17934517
NUT midline carcinoma of the parotid gland with mesenchymal differentiation. den Bakker MA, Beverloo BH, van den Heuvel-Eibrink MM, Meeuwis CA, Tan LM, Johnson LA, French CA, van Leenders GJ. Am J Surg Pathol. 2009 Aug;33(8):1253-8. PMID: 19561446
NUT rearrangement in undifferentiated carcinomas of the upper aerodigestive tract. Stelow EB, Bellizzi AM, Taneja K, Mills SE, Legallo RD, Kutok JL, Aster JC, French CA. Am J Surg Pathol. 2008 Jun;32(6):828-34. PMID: 18391746
Recurrent fusion oncogenes in carcinomas. Teixeira MR. Crit Rev Oncog. 2006 Dec;12(3-4):257-71. PMID: 17425505
Midline carcinoma with t(15;19) and BRD4-NUT fusion oncogene in a 30-year-old female with response to docetaxel and radiotherapy. Engleson J, Soller M, Panagopoulos I, Dahlén A, Dictor M, Jerkeman M. BMC Cancer. 2006 Mar 16;6:69. PMID: 16542442
Successful treatment of a child with t(15;19)-positive tumor. Mertens F, Wiebe T, Adlercreutz C, Mandahl N, French CA. Pediatr Blood Cancer. 2007 Dec;49(7):1015-7. PMID: 16435379
Cloned fusion product from a rare t(15;19)(q13.2;p13.1) inhibit S phase in vitro. Haruki N, Kawaguchi KS, Eichenberger S, Massion PP, Gonzalez A, Gazdar AF, Minna JD, Carbone DP, Dang TP. J Med Genet. 2005 Jul;42(7):558-64. PMID: 15994877
Midline carcinoma of children and young adults with NUT rearrangement. French CA, Kutok JL, Faquin WC, Toretsky JA, Antonescu CR, Griffin CA, Nose V, Vargas SO, Moschovi M, Tzortzatou-Stathopoulou F, Miyoshi I, Perez-Atayde AR, Aster JC, Fletcher JA. J Clin Oncol. 2004 Oct 15;22(20):4135-9. PMID: 15483023
BRD4-NUT fusion oncogene: a novel mechanism in aggressive carcinoma. French CA, Miyoshi I, Kubonishi I, Grier HE, Perez-Atayde AR, Fletcher JA. Cancer Res. 2003 Jan 15;63(2):304-7. PMID: 12543779
The cytologic features of NUT midline carcinoma. Bellizzi AM, Bruzzi C, French CA, Stelow EB. Cancer Cytopathol. 2009 Sep 30. PMID: 19795508
NUT midline carcinoma of the parotid gland with mesenchymal differentiation. den Bakker MA, Beverloo BH, van den Heuvel-Eibrink MM, Meeuwis CA, Tan LM, Johnson LA, French CA, van Leenders GJ. Am J Surg Pathol. 2009 Aug;33(8):1253-8. PMID: 19561446
Carcinomas of the upper aerodigestive tract with rearrangement of the nuclear protein of the testis (NUT) gene (NUT midline carcinomas). Stelow EB, French CA. Adv Anat Pathol. 2009 Mar;16(2):92-6. PMID: 19550370
Diagnosis of NUT Midline Carcinoma Using a NUT-specific Monoclonal Antibody. Haack H, Johnson LA, Fry CJ, Crosby K, Polakiewicz RD, Stelow EB, Hong SM, Schwartz BE, Cameron MJ, Rubin MA, Chang MC, Aster JC, French CA. Am J Surg Pathol. 2009 Jul;33(7):984-91. PMID: 19363441
Molecular pathology of NUT midline carcinomas. French CA. J Clin Pathol. 2008 Jun 13. PMID: 18552174
NUT rearrangement in undifferentiated carcinomas of the upper aerodigestive tract. Stelow EB, Bellizzi AM, Taneja K, Mills SE, Legallo RD, Kutok JL, Aster JC, French CA. Am J Surg Pathol. 2008 Jun;32(6):828-34. PMID: 18391746
BRD-NUT oncoproteins: a family of closely related nuclear proteins that block epithelial differentiation and maintain the growth of carcinoma cells. French CA, Ramirez CL, Kolmakova J, Hickman TT, Cameron MJ, Thyne ME, Kutok JL, Toretsky JA, Tadavarthy AK, Kees UR, Fletcher JA, Aster JC. Oncogene. 2008 Apr 3;27(15):2237-42. PMID: 17934517
Recurrent fusion oncogenes in carcinomas. Teixeira MR. Crit Rev Oncog. 2006 Dec;12(3-4):257-71. PMID: 17425505
Midline carcinoma with t(15;19) and BRD4-NUT fusion oncogene in a 30-year-old female with response to docetaxel and radiotherapy. Engleson J, Soller M, Panagopoulos I, Dahlén A, Dictor M, Jerkeman M. BMC Cancer. 2006 Mar 16;6:69. PMID: 16542442
Midline carcinoma of children and young adults with NUT rearrangement. French CA, Kutok JL, Faquin WC, Toretsky JA, Antonescu CR, Griffin CA, Nose V, Vargas SO, Moschovi M, Tzortzatou-Stathopoulou F, Miyoshi I, Perez-Atayde AR, Aster JC, Fletcher JA. J Clin Oncol. 2004 Oct 15;22(20):4135-9. PMID: 15483023
BRD4-NUT fusion oncogene: a novel mechanism in aggressive carcinoma. French CA, Miyoshi I, Kubonishi I, Grier HE, Perez-Atayde AR, Fletcher JA. Cancer Res. 2003 Jan 15;63(2):304-7. PMID: 12543779