Mutation-specific IHC

HRAS-Q61R IHC
Open references
NRASQ61R Mutation-specific Immunohistochemistry Also Identifies the HRASQ61R Mutation in Medullary Thyroid Cancer and May Have a Role in Triaging Genetic Testing for MEN2. Reagh J, Bullock M, Andrici J, Turchini J, Sioson L, Clarkson A, Watson N, Sheen A, Lim G, Delbridge L, Sidhu S, Sywak M, Aniss A, Shepherd P, Ng D, Oei P, Field M, Learoyd D, Robinson BG, Clifton-Bligh RJ, Gill AJ. Am J Surg Pathol. 2016 Sep 15. PMID: #27635947# (...)

IHC for BRAF V600E is relatively sensitive and specific, but there is a discordance rate that is not trivial with NGS mutation profiling. In addition, a significant proportion of patients harbor BRAF non-V600E not detectable by IHC, potentially limiting utility of IHC screening for BRAF mutations. (#25634750#)
References
NRASQ61R and BRAFV600E Mutation-specific Immunohistochemistry Is a Helpful Tool to Diagnose Metastatic Undifferentiated/Dedifferentiated Melanomas: Agaimy A, Mentzel T. (...)