Live imaging

• live-cell imaging-based RNAi screen
  22 October 2011

  References
  RNAi-based screening identifies the Mms22L-Nfkbil2 complex as a novel regulator of DNA replication in human cells. Piwko W, Olma MH, Held M, Bianco JN, Pedrioli PG, Hofmann K, Pasero P, Gerlich DW, Peter M. EMBO J. 2010 Dec 15;29(24):4210-22. PMID: #21113133#

• high-throughput live cell imaging
  22 October 2011

  References
  CellCognition: time-resolved phenotype annotation in high-throughput live cell imaging. Held M, Schmitz MH, Fischer B, Walter T, Neumann B, Olma MH, Peter M, Ellenberg J, Gerlich DW. Nat Methods. 2010 Sep;7(9):747-54. PMID: #20693996#

• intravital imaging
  22 October 2011

  Intravital imaging using fluorescent proteins
  Before the introduction of fluorescent proteins, in vivo imaging was limited to the study of cells that were transiently labelled with vital dyes.
  Stable fluorescent labelling, achieved using vectors that express fluorescent proteins, now allows the direct imaging of single cells in vivo.
  Using what is termed intravital microscopy — observation of a tumour of interest either through surgically created chronic-transparent windows or directly (...)

• super-resolution imaging in living cells
  22 October 2011

  super-resolution imaging in live cells; live cell super-resolution imaging

• calcium imaging
  22 September 2008

  Calcium imaging has revolutionized the approaches for functional analyses in the living brain of animal experimental models.
  Changes in intracellular calcium concentration are strictly linked to the electrical activity in neurons and produce signals that are effectively detected by optical methods.
  Distinctive features of fluorescence-based calcium imaging are its high temporal resolution in the millisecond range and its high spatial resolution in the micrometer range.
  Recent progress (...)

• in vivo pathology
  14 July 2008

  References
  Contag CH. In vivo pathology: seeing with molecular specificity and cellular resolution in the living body. Annu Rev Pathol. 2007;2:277-305. PMID: #18039101#

• fluorescence complementation
  22 November 2006

  The visualization of protein complexes in living cells enables the examination of protein interactions in their normal environment and the determination of their subcellular localization.
  The bimolecular fluorescence complementation assay has been used to visualize interactions among multiple proteins in many cell types and organisms.
  See also
  competition between alternative interaction partners
  covalent modification of proteins by ubiquitin-family peptides.
  References
  Kerppola TK. (...)

• photoactivatable fluorescent proteins
  30 January 2006

  References
  Hoffman RM. The multiple uses of fluorescent proteins to visualize cancer in vivo. Nat Rev Cancer. 2005 Oct;5(10):796-806. PMID: #16195751#
  Lukyanov KA, Chudakov DM, Lukyanov S, Verkhusha VV. Innovation: Photoactivatable fluorescent proteins. Nat Rev Mol Cell Biol. 2005 Nov;6(11):885-91. PMID: #16167053#

• fluorescence molecular imaging
  30 August 2005

  References
  Graves EE, Weissleder R, Ntziachristos V. Fluorescence molecular imaging of small animal tumor models. Curr Mol Med. 2004 Jun;4(4):419-30. PMID: #15354872#

• in vivo cell imaging
  20 April 2004

  in vivo microscopy

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