D2-40

Definition: D2-40 is a monoclonal antibody recognizing podoplanin , a 40 000 kD O-linked sialoglycoprotein located in lymphatic endothelial cells, coded by gene PDPN . This oncofetal antigen is present in fetal germ cells.

Images

 A vascular tumor of kaposiform hemangioendothelioma/tufted angioma spectrum in an infant. Note the characteristic podoplanin / PDPN stain

• https://twitter.com/ikletskaya/status/822082053814493184

Expression in normal cells

 endothelium of lymphatic channels

Expression in tumors

 lymphangiomas
 kaposiform hemangioendothelioma (KHE)
 Kaposi sarcoma (KS)
 benign tumors of lymphatic origin

• Hobnail hemangiomas (targetoid hemosiderotic hemangiomas) (THH) (15059220)

 epithelioid malignant mesothelioma (86%) (15891998)
 angiosarcoma (40%) (15891998)

Negative tumors

 hemangiomas
 glomus tumors
 angiolipomas
 pyogenic granuloma
  vascular malformations
 hemangiopericytoma
 hemangioendothelioma

Pathology

 D2-40 might be useful to detect missed hematoxylin-eosin emboli especially in cases without any other prognostically unfavorable criterion. (18820667)

See also

 O-linked sialoglycoprotein

References

 de Mascarel I, Macgrogan G, Debled M, Sierankowski G, Brouste V, Mathoulin-Pélissier S, Mauriac L. D2-40 in breast cancer: should we detect more vascular emboli? Mod Pathol. 2008 Sep 26. PMID: 18820667

 Debelenko LV, Perez-Atayde AR, Mulliken JB, Liang MG, Archibald TH, Kozakewich HP. D2-40 immunohistochemical analysis of pediatric vascular tumors reveals positivity in kaposiform hemangioendothelioma. Mod Pathol. 2005 Nov;18(11):1454-60. PMID: 15920541

 Roy S, Chu A, Trojanowski JQ, Zhang PJ. D2-40, a novel monoclonal antibody against the M2A antigen as a marker to distinguish hemangioblastomas from renal cell carcinomas.
Acta Neuropathol (Berl). 2005 May;109(5):497-502. PMID: 15864611

 Hornick JL, Fletcher CD. Intraabdominal cystic lymphangiomas obscured by marked superimposed reactive changes: clinicopathological analysis of a series. Hum Pathol. 2005 Apr;36(4):426-32. PMID: 15892005

 Ordonez NG. D2-40 and podoplanin are highly specific and sensitive immunohistochemical markers of epithelioid malignant mesothelioma. Hum Pathol. 2005 Apr;36(4):372-80. PMID: 15891998

 Fukunaga M. Expression of D2-40 in lymphatic endothelium of normal tissues and in vascular tumours. Histopathology. 2005 Apr;46(4):396-402. PMID: 15810951

 Kahn HJ, Bailey D, Marks A. Monoclonal antibody D2-40, a new marker of lymphatic endothelium, reacts with Kaposi sarcoma and a subset of angiosarcomas. Mod Pathol. 2002 Apr;15(4):434-40. PMID: 11950918