RNA splicing

Spicing modifications

Splicing can be experimentally modified so that targeted exons are excluded from mature mRNA transcripts by blocking the access of splice-directing small nuclear ribonucleoprotein particles (snRNPs) to pre-mRNA using Morpholino antisense oligos. This has become a standard technique in developmental biology. Morpholino oligos can also be targeted to prevent molecules that regulate splicing (e.g. splice enhancers, splice suppressors) from binding to pre-mRNA, altering patterns of splicing.

References

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 Nissim-Rafinia M, Kerem B. The splicing machinery is a genetic modifier of disease severity. Trends Genet. 2005 Sep;21(9):480-3. PMID: 16039004

 Barrass JD, Beggs JD. Splicing goes global. Trends Genet. 2003 Jun;19(6):295-8. PMID: 12801718

 Faustino NA, Cooper TA. Pre-mRNA splicing and human disease. Genes Dev. 2003 Feb 15;17(4):419-37. PMID: 12600935

 Cartegni L, Chew SL, Krainer AR. Listening to silence and understanding nonsense: exonic mutations that affect splicing. Nat Rev Genet. 2002 Apr;3(4):285-98. PMID: 11967553

 Stamm S. Signals and their transduction pathways regulating alternative splicing : a new dimension of the human genome. Hum Mol Genet. 2002 Oct 1 ;11(20):2409-16. PMID : 12351576

 Nissim-Rafinia M, Kerem B. Splicing regulation as a potential genetic modifier. Trends Genet. 2002 Mar;18(3):123-7. PMID: 11858835