Place the proteins

A central aim of cell biology is to find out where each protein functions in the cell.

In yeast

In 2003, 75% of the yeast proteome was known. In 2003, two large-scale studies of Saccharomyces cerevisiae, involving labelling over 4,000 proteins with GFP fluorescence or TAP (tandem affinity purification) tags, have made it possible to determine which proteins are made and how many copies per cell, and to track down their subcellular locations, such as the nucleus, nucleolus, mitochondria and the Golgi apparatus. The database, and strain collection information are accessible at http://yeastgfp.ucsf.edu.

See also

 high-throughput protein arrays
 organelle proteomics

References

 Brunet S, Thibault P, Gagnon E, Kearney P, Bergeron JJ, Desjardins M. Organelle proteomics: looking at less to see more. Trends Cell Biol. 2003 Dec;13(12):629-38. PMID: #14624841#

 Baak JP, Path FR, Hermsen MA, Meijer G, Schmidt J, Janssen EA. Genomics and proteomics in cancer. Eur J Cancer. 2003 Jun;39(9):1199-215. PMID: #12763207#

 Wulfkuhle JD, Liotta LA, Petricoin EF. Proteomic applications for the early detection of cancer. Nat Rev Cancer. 2003 Apr;3(4):267-75. PMID: #12671665#

 Bader GD, Heilbut A, Andrews B, Tyers M, Hughes T, Boone C. Functional genomics and proteomics: charting a multidimensional map of the yeast cell. Trends Cell Biol. 2003 Jul;13(7):344-56. PMID: #12837605#

 Brunet S, Thibault P, Gagnon E, Kearney P, Bergeron JJ, Desjardins M. Organelle proteomics: looking at less to see more. Trends Cell Biol. 2003 Dec;13(12):629-38. PMID: #14624841#

 MacBeath G. Protein microarrays and proteomics. Nat Genet. 2002 Dec;32 Suppl:526-32. PMID: #12454649#