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RNA interference

Sunday 13 July 2003

- In post-transcriptional gene silencing, double-stranded RNA triggers are processed to small interfering RNAs, which act to seek out and destroy homologous transcripts.

- A variety of organisms utilise the RNAi pathway to silence expression of potentially harmful endogenous mobile elements and to eliminate unnecessary sequences.

In plants and fission yeast, RNAi can also mediate chromatin-based silencing resulting in transcriptional shutdown of homologous transcription units (transcriptional gene silencing) and the formation of centromeric heterochromatin.

In metazoans, the expression of non-coding RNAs is often associated with the formation of silent chromatin domains but it remains to be determined if RNAi is involved.

- RNA interference (RNAi) is widely used for knocking down expression of genes of interest and in systematic screens for desired phenotypes.

See also

- RNAi screening

Videos

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References

- Nishikura K. Editor meets silencer: crosstalk between RNA editing and RNA interference. Nat Rev Mol Cell Biol. 2006 Dec;7(12):919-31. PMID: 17139332

- Echeverri CJ, Perrimon N. High-throughput RNAi screening in cultured cells: a user’s guide. Nat Rev Genet. 2006 May;7(5):373-84. PMID: 16607398

- Buckingham SD, Esmaeili B, Wood M, Sattelle DB. RNA interference: from model organisms towards therapy for neural and neuromuscular disorders. Hum Mol Genet. 2004 Oct 1;13 Spec No 2:R275-88. PMID: 15358735

- Matzke MA, Birchler JA. RNAi-mediated pathways in the nucleus. Nat Rev Genet. 2005 Jan;6(1):24-35. PMID: 15630419

- Stevenson M. Therapeutic potential of RNA interference. N Engl J Med. 2004 Oct 21;351(17):1772-7. PMID: 15496626

- Buckingham SD, Esmaeili B, Wood M, Sattelle DB. RNA interference: from model organisms towards therapy for neural and neuromuscular disorders. Hum Mol Genet. 2004 Oct 1;13 Suppl 2:R275-88. PMID: 15358735

- Schramke V, Allshire R. Those interfering little RNAs! Silencing and eliminating chromatin. Curr Opin Genet Dev. 2004 Apr;14(2):174-80. PMID: 15196464

- Mittal V. Improving the efficiency of RNA interference in mammals. Nat Rev Genet. 2004 May;5(5):355-65. PMID: 15143318

- Lieberman J, Song E, Lee SK, Shankar P. Interfering with disease: opportunities and roadblocks to harnessing RNA interference. Trends Mol Med. 2003 Sep;9(9):397-403. PMID: 13129706

- Kitabwalla M, Ruprecht RM. RNA interference—a new weapon against HIV and beyond. N Engl J Med. 2002 Oct 24;347(17):1364-7. PMID: 12397198

- Silva JM, Hammond SM, Hannon GJ. RNA interference: a promising approach to antiviral therapy? Trends Mol Med. 2002 Nov;8(11):505-8. PMID: 12421679

- McManus MT, Sharp PA. Gene silencing in mammals by small interfering RNAs. Nat Rev Genet. 2002 Oct;3(10):737-47. PMID: 12360232