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mitotic spindle

Tuesday 24 August 2004

Kinetochores are large protein complexes that are formed on chromosome regions known as centromeres. For high-fidelity chromosome segregation, kinetochores must be correctly captured on the mitotic spindle before anaphase onset.

During prometaphase, kinetochores are initially captured by a single microtubule that extends from a spindle pole and are then transported poleward along the microtubule.

Subsequently, microtubules that extend from the other spindle pole also interact with kinetochores and, eventually, each sister kinetochore attaches to microtubules that extend from opposite poles - this is known as bi-orientation.

The spindle assembly checkpoint (SAC) ensures the fidelity of chromosome segregation by preventing cell-cycle progression until all the chromosomes make proper bipolar attachments to the mitotic spindle and come under tension.

See also

- mitosis


- Musacchio A, Salmon ED. The spindle-assembly checkpoint in space and time. Nat Rev Mol Cell Biol. 2007 May;8(5):379-93. PMID: 17426725

- McCarthy EK, Goldstein B. Asymmetric spindle positioning. Curr Opin Cell Biol. 2006 Feb;18(1):79-85. PMID: 16361093

- Tanaka TU, Stark MJ, Tanaka K. Kinetochore capture and bi-orientation on the mitotic spindle. Nat Rev Mol Cell Biol. 2005 Dec;6(12):929-42. PMID: #16341079

- Pinsky BA, Biggins S. The spindle checkpoint: tension versus attachment. Trends Cell Biol. 2005 Sep;15(9):486-93. PMID: 16084093

- Vanoosthuyse V, Hardwick KG. Bub1 and the multilayered inhibition of Cdc20-APC/C in mitosis. Trends Cell Biol. 2005 May;15(5):231-3. PMID: 15866025

- Rieder CL, Maiato H. Stuck in division or passing through: what happens when cells cannot satisfy the spindle assembly checkpoint. Dev Cell. 2004 Nov;7(5):637-51. PMID: 15525526

- The mechanism of spindle assembly: functions of Ran and its target TPX2. J Cell Biol. 2004 Sep 27;166(7):949-55. PMID: 15452138

- Gadde S, Heald R. Mechanisms and molecules of the mitotic spindle. Curr Biol. 2004 Sep 21;14(18):R797-805. PMID: 15380094

- Wadsworth P, Khodjakov A. E pluribus unum: towards a universal mechanism for spindle assembly. Trends Cell Biol. 2004 Aug;14(8):413-9. PMID: 15308207