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Home > Technical section > Immunochemistry > Diagnostic antibodies > D2-40


Tuesday 24 August 2004

Definition: D2-40 is a monoclonal antibody recognizing podoplanin , a 40 000 kD O-linked sialoglycoprotein located in lymphatic endothelial cells, coded by gene PDPN . This oncofetal antigen is present in fetal germ cells.


- A vascular tumor of kaposiform hemangioendothelioma/tufted angioma spectrum in an infant. Note the characteristic podoplanin / PDPN stain

Expression in normal cells

- endothelium of lymphatic channels

Expression in tumors

- lymphangiomas
- kaposiform hemangioendothelioma (KHE)
- Kaposi sarcoma (KS)
- benign tumors of lymphatic origin

- epithelioid malignant mesothelioma (86%) (15891998)
- angiosarcoma (40%) (15891998)

Negative tumors

- hemangiomas
- glomus tumors
- angiolipomas
- pyogenic granuloma
-  vascular malformations
- hemangiopericytoma
- hemangioendothelioma


- D2-40 might be useful to detect missed hematoxylin-eosin emboli especially in cases without any other prognostically unfavorable criterion. (18820667)

See also

- O-linked sialoglycoprotein


- de Mascarel I, Macgrogan G, Debled M, Sierankowski G, Brouste V, Mathoulin-PĂ©lissier S, Mauriac L. D2-40 in breast cancer: should we detect more vascular emboli? Mod Pathol. 2008 Sep 26. PMID: 18820667

- Debelenko LV, Perez-Atayde AR, Mulliken JB, Liang MG, Archibald TH, Kozakewich HP. D2-40 immunohistochemical analysis of pediatric vascular tumors reveals positivity in kaposiform hemangioendothelioma. Mod Pathol. 2005 Nov;18(11):1454-60. PMID: 15920541

- Roy S, Chu A, Trojanowski JQ, Zhang PJ. D2-40, a novel monoclonal antibody against the M2A antigen as a marker to distinguish hemangioblastomas from renal cell carcinomas.
Acta Neuropathol (Berl). 2005 May;109(5):497-502. PMID: 15864611

- Hornick JL, Fletcher CD. Intraabdominal cystic lymphangiomas obscured by marked superimposed reactive changes: clinicopathological analysis of a series. Hum Pathol. 2005 Apr;36(4):426-32. PMID: 15892005

- Ordonez NG. D2-40 and podoplanin are highly specific and sensitive immunohistochemical markers of epithelioid malignant mesothelioma. Hum Pathol. 2005 Apr;36(4):372-80. PMID: 15891998

- Fukunaga M. Expression of D2-40 in lymphatic endothelium of normal tissues and in vascular tumours. Histopathology. 2005 Apr;46(4):396-402. PMID: 15810951

- Kahn HJ, Bailey D, Marks A. Monoclonal antibody D2-40, a new marker of lymphatic endothelium, reacts with Kaposi sarcoma and a subset of angiosarcomas. Mod Pathol. 2002 Apr;15(4):434-40. PMID: 11950918