Sunday 18 July 2004
RNA interference (RNAi) is triggered by double-stranded RNA helices that have been introduced exogenously into cells as small interfering (si)RNAs or that have been produced endogenously from small non-coding RNAs known as microRNAs (miRNAs).
Small RNAs are key regulators of mRNA turnover and translation. The small RNAs termed microRNAs (miRNAS) play important roles in cell proliferation, apoptosis and differentiation. MicroRNA mechanism is an efficient means to regulate production of a diverse range of proteins. RNA-based regulation of mRNAs may rival ubiquitination as a mechanism to control protein levels.
RNAi has become a standard experimental tool and its therapeutic potential is being aggressively harnessed. Understanding the structure and function of small RNAs, such as siRNAs and miRNAs, that trigger RNAi has shed light on the RNAi machinery.
In particular, it has highlighted the assembly and function of the RNA-induced silencing complex (RISC), and has provided guidelines to efficiently silence genes for biological research and therapeutic applications of RNAi.
- miRNAs (microRNAs)
Development: Transposon-derived small RNAs control patterning.
Muers M. Nat Rev Genet. 2010 Dec;11(12):816. PMID: 21045867
Small RNA sorting: matchmaking for Argonautes. Czech B, Hannon GJ. Nat Rev Genet. 2011 Jan;12(1):19-31. PMID: 21116305
Chapman EJ, Carrington JC. Specialization and evolution of endogenous small RNA pathways. Nat Rev Genet. 2007 Nov;8(11):884-96. PMID: 17943195
Rana TM. Illuminating the silence: understanding the structure and function of small RNAs. Nat Rev Mol Cell Biol. 2007 Jan;8(1):23-36. PMID: 17183358
Mattick JS, Makunin IV. Small regulatory RNAs in mammals. Hum Mol Genet. 2005 Apr 15;14 Spec No 1:R121-32. PMID: 15809264
Nakahara K, Carthew RW. Expanding roles for miRNAs and siRNAs in cell regulation. Curr Opin Cell Biol. 2004 Apr;16(2):127-33. PMID: 15196554