Sunday 5 January 2014
Ensemble-decision aliquot ranking
Several technologies recently have been developed for separating and counting circulating tumor cells (CTCs) in the human blood. CTCs play an important role in the metastasis of cancer.
Most of the current applications are focused on the enumeration of CTCs; however, analysis of the enumerated CTCs has been proven to be increasingly important.
eDAR : Ensemble-decision aliquot ranking
- Ensemble-decision aliquot ranking (eDAR) is a high-throughput method that allows the isolation of the CTCs from the whole blood with high recovery and a zero false-positive rate.
- Coupling a CTC separation and capturing method, such as eDAR, with a downstream immunostaining test provides information about the cell’s expression of certain protein biomarkers.
- eDAR is a semi-automated system for sequential immunolabeling and photobleaching on the eDAR platform.
- With this technique, it is possible to evaluate the expression of eight different biomarkers on isolated CTCs.
Sensitive and high-throughput isolation of rare cells from peripheral blood with ensemble-decision aliquot ranking. Schiro PG, Zhao M, Kuo JS, Koehler KM, Sabath DE, Chiu DT. Angew Chem Int Ed Engl. 2012 May 7;51(19):4618-22. doi:10.1002/anie.201108695 . PMID: #22359315# [Free]
Imaging multiple biomarkers in captured rare cells by sequential immunostaining and photobleaching. Zhao M, Wei B, Chiu DT. Methods. 2013 Dec 1;64(2):108-13. doi:10.1016/j.ymeth.2013.08.006 . PMID: #23954571#