Sunday 5 January 2014
Ensemble-decision aliquot ranking
Most of the current applications are focused on the enumeration of CTCs; however, analysis of the enumerated CTCs has been proven to be increasingly important.
eDAR : Ensemble-decision aliquot ranking
- Ensemble-decision aliquot ranking (eDAR) is a high-throughput method that allows the isolation of the CTCs from the whole blood with high recovery and a zero false-positive rate.
- Coupling a CTC separation and capturing method, such as eDAR, with a downstream immunostaining test provides information about the cell’s expression of certain protein biomarkers.
- eDAR is a semi-automated system for sequential immunolabeling and photobleaching on the eDAR platform.
- With this technique, it is possible to evaluate the expression of eight different biomarkers on isolated CTCs.
Sensitive and high-throughput isolation of rare cells from peripheral blood with ensemble-decision aliquot ranking. Schiro PG, Zhao M, Kuo JS, Koehler KM, Sabath DE, Chiu DT. Angew Chem Int Ed Engl. 2012 May 7;51(19):4618-22. doi : 10.1002/anie.201108695 PMID: 22359315 [Free]
Imaging multiple biomarkers in captured rare cells by sequential immunostaining and photobleaching. Zhao M, Wei B, Chiu DT. Methods. 2013 Dec 1;64(2):108-13. doi : 10.1016/j.ymeth.2013.08.006 PMID: 23954571