Sunday 5 January 2014
Most of the current applications are focused on the enumeration of CTCs; however, analysis of the enumerated CTCs has been proven to be increasingly important.
- Ensemble-decision aliquot ranking (eDAR) is a high-throughput method that allows the isolation of the CTCs from the whole blood with high recovery and a zero false-positive rate.
- Coupling a CTC separation and capturing method, such as eDAR, with a downstream immunostaining test provides information about the cell’s expression of certain protein biomarkers.
- eDAR is a semi-automated system for sequential immunolabeling and photobleaching on the eDAR platform.
- With this technique, it is possible to evaluate the expression of eight different biomarkers on isolated CTCs.
Imaging multiple biomarkers in captured rare cells by sequential immunostaining and photobleaching. Zhao M, Wei B, Chiu DT. Methods. 2013 Dec 1;64(2):108-13. doi : 10.1016/j.ymeth.2013.08.006 PMID: 23954571