exosome in cancer
Sunday 5 January 2014
Exosomes are small membrane vesicles released by most cell types including tumor cells. The intercellular exchange of proteins and genetic material via exosomes is a potentially effective approach for cell-to-cell communication and it may perform multiple functions aiding to tumor survival and metastasis.
Exosome in cancer
- The exosome has been shown to be inhibited by the antimetabolite drug fluorouracil, which is a drug used in chemotherapy treatment of cancer. It is one of the most successful drugs for treating solid tumors.
- In yeast cells treated with fluorouracil, defects were seen in the processing of ribosomal RNA identical to those seen when the activity of the exosome was blocked by molecular biological strategies.
- Lack of correct ribosomal RNA processing is lethal to cells, explaining the antimetabolic effect of the drug.
- MicroRNA and protein profiling of brain metastasis competent cell-derived exosomes. doi:10.1371/journal.pone.0073790 .PMID: #24066071#
It has been developed working procedures to evaluate exosomal RNA signature as novel biomarkers for cancer prognosis, prediction and patient stratification.
In particular, it has refreshed current practice and demonstrated a new approach for studying RNA signature in patient blood samples.
MicroRNA and protein profiling of brain metastasis competent cell-derived exosomes. Camacho L, Guerrero P, Marchetti D. PLoS One. 2013 Sep 16;8(9):e73790. doi:10.1371/journal.pone.0073790 . PMID: #24066071# (Free)
Proteomic profiling of exosomes leads to the identification of novel biomarkers for prostate cancer. Duijvesz D, Burnum-Johnson KE, Gritsenko MA, Hoogland AM, Vredenbregt-van den Berg MS, Willemsen R, Luider T, Paša-Tolić L, Jenster G. PLoS One. 2013 Dec 31;8(12):e82589. doi:10.1371/journal.pone.0082589 . PMID: #24391718# [Free]
The roles of extracellular vesicles in cancer biology: towards the development of novel cancer biomarkers. Katsuda T, Kosaka N, Ochiya T. Proteomics. 2013 Dec 12. doi:10.1002/pmic.201300389 . PMID: #24339442#
Prostate Cancer Biomarker Profiles in Urinary Sediments and Exosomes. Dijkstra S, Birker IL, Smit FP, Leyten GH, de Reijke TM, van Oort IM, Mulders PF, Jannink SA, Schalken JA. J Urol. 2013 Nov 7. pii: S0022-5347(13)05884-9. doi:10.1016/j.juro.2013.11.001 . PMID: #24211598#
Clinical use of novel urine and blood based prostate cancer biomarkers: A review. Dijkstra S, Mulders PF, Schalken JA. Clin Biochem. 2013 Oct 29. pii: S0009-9120(13)00497-9. doi: 10.1016/j.clinbiochem.2013.10.023. PMID: #24177197#