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Anti-Cytokeratin (CAM 5.2)

Tuesday 12 February 2013

Anti-Cytokeratin (CAM 5.2) reagent has a primary reactivity with human keratin proteins that correspond to Moll’s peptides #7 and #8, Mr 48 and 52 kilodaltons (kd), respectively.

Cytokeratin 7 and 8 are present on secretory epithelia of normal human tissue but not on
stratified squamous epithelium.

Anti-Cytokeratin (CAM 5.2) stains most epithelial-derived tissue, including liver, renal tubular epithelium, and hepatocellular and renal cell carcinomas.

Anti-Cytokeratin (CAM 5.2) might not react with some squamous cell carcinomas.

References

- Moll R, Franke W, Schiller D, Geiger B, Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors, and cultured cells. Cell 1982;31:11-24.
- Smedts F, Ramaekers F, Robben H, et al. Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am J Pathol. 1990;136(3):657-667.
- Makin C, Bobrow L, Bodmer W. Monoclonal antibody to cytokeratin for use in routine histopathology. J Clin Pathol 1984;37:975.
- Cooper D, Schermer A, Sun T-T. Biology of disease classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications and limitations. Lab Invest. 1985;52:243-256.
- Battifora H. Diagnostic uses of antibodies to keratins: a review and immunohistochemical comparison of seven monoclonal and three polyclonal antibodies. Prog Surg Pathol. 1990;8:1-15.
- Leader M, Patel J, Makin C, Henry K. An analysis of the sensitivity and specificity of the cytokeratin marker CAM 5.2 for epithelial tumors: results of a study of 203 sarcomas, 50 carcinomas, and 28 malignant melanomas.Histopathol 1986;10:1315-1324.