impact of thawing
Wednesday 7 December 2011
Modified primer design or the use of different housekeeping genes could not rectify the changes for FOS.
Repetitive thawing cycles had similar effects on RNA integrity.
The incubation of the tissue in RNAlater efficiently prevented RNA degradation.
In conclusion, degradation of RNA in frozen tissue occurs first after several minutes of thawing.
Already minimal decrease in RNA quality may result in significant changes in gene expression patterns in clinical tissue samples.
Impact of thawing on RNA integrity and gene expression analysis in fresh frozen tissue. Botling J, Edlund K, Segersten U, Tahmasebpoor S, Engström M, Sundström M, Malmström PU, Micke P. Diagn Mol Pathol. 2009 Mar;18(1):44-52. PMID: 19214109