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fluorescence polarization assay

Friday 25 November 2011

Currently, the most used methods for genotyping of SNPs are costly and time consuming. It is necessary to develop a method that is more accurate, cost-effective, and simple.

An improved fluorescence polarization assay based on asymmetric polymerase chain reaction hybridization for screening SNPs has been developed.

Studies show the accuracy, simplicity, and cost-effectiveness of the fluorescence polarization assay in the detection of SNPs.

In a study, the fluorescence polarization assay is more accurate for the heterozygous sample than is the sequence assay. The minimum detection level established with the fluorescence polarization assay is 2.5 genome copies per reaction and no cross-reaction is observed. (20736746)

References

- Detection of ERCC1 118 polymorphisms in non-small-cell lung cancer by an improved fluorescence polarization assay. Wenchao L, Xiaohui L, Yan X, Helong Z, Wenzhao Y, Yanhai G, Zhen Y, Ju Z. Diagn Mol Pathol. 2010 Sep;19(3):164-8. PMID: 20736746

- Detection of p16INK4a promoter methylation status in non-small cell lung cancer by a fluorescence polarization assay. Song Z, Zhou R, Li D, Chen Y, Liang P, Liu W, Zhang J. Diagn Mol Pathol. 2011 Sep;20(3):138-42. PMID: 21817906