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adipose-derived stem cells

Monday 17 October 2011

When adipose-derived stem cells (ASCs) are retrieved from the stromal vascular portion of adipose tissue, a large amount of mature adipocytes are often discarded.

However, by modified ceiling culture technique based on their buoyancy, mature adipocytes can be easily isolated from the adipose cell suspension and dedifferentiated into lipid-free fibroblast-like cells, named dedifferentiated fat (DFAT) cells.

DFAT cells re-establish active proliferation ability and undertake multipotent capacities.

Compared with ASCs and other adult stem cells, DFAT cells showed unique advantages in their abundance, isolation and homogeneity.

Under proper induction culture in vitro or environment in vivo, DFAT cells could demonstrate adipogenic, osteogenic, chondrogenic and myogenic potentials.

In angiogenic conditions, DFAT cells could exhibit perivascular characteristics and elicit neovascularization.

Current research on DFAT cells indicated that this alternative source of adult multipotent cells has great potential in tissue engineering and regenerative medicine.

See also

- mesenchymal stem cells
- tissue-specific stem cells

References

- Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues. Shen JF, Sugawara A, Yamashita J, Ogura H, Sato S. Int J Oral Sci. 2011 Jul;3(3):117-24. PMID: 21789960 [Free]

- Adipose-derived stem cells: current findings and future perspectives. Tobita M, Orbay H, Mizuno H. Discov Med. 2011 Feb;11(57):160-70. PMID: 21356171 [Free]