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DPEP1

16q24 HGNC:3002 MIM.179780

Friday 14 January 2011

Immunochemistry

- DPEP1 expression in colorectal adenocarcinoma (20824289)

  • DPEP1 is expressed in the early stages of colon carcinogenesis.
  • DPEP1 affects cancer cell invasiveness. (20824289)
  • The Cancer Genome Anatomy Project was used to detect differences in gene expression between normal and cancer tissue.
  • Immunohistochemical analysis indicated that 13/16 ovarian metastases of colorectal cancers, but only 1/58 primary mucinous ovarian cancers, were dipeptidase 1-positive (threshold; ≧25% expression, P@<@0.0001).
  • The overexpression of dipeptidase-1 (DPEP1) in cancer tissue was confirmed in a sample of 76 patients by real-time PCR.
  • To identify the function of DPEP1, RNA interference (RNAi) was used to inactivate this gene in the colon cancer cell line.
  • Immunohistochemical analysis was performed to characterize the pattern of DPEP1 expression in colon cancer. (20824289)
  • DPEP1 expression in cancer is significantly higher than that in normal tissue.
  • DPEP1 expression decreased with pathological differentiation, lymph-node and distant metastasis. (20824289)
  • Patients with tumors with decreased DPEP1 expression showed a poorer prognosis, and this was also true of patients with tumors who are treated with curative intent. (20824289)
  • RNAi-mediated DPEP1 reduction in the colon cancer cell line did not result in cell proliferation or apoptosis, but was associated with an increased invasive ability. (20824289)
  • DPEP1 protein was observed on the apical side of the cancer cells, and is expressed in the early stages of carcinogenesis, even in adenomas of both sporadic colorectal cancer and familial adenomatous polyposis patients. (20824289)
  • DPEP1 expression in normal colonic mucosa is very low, but it is highly expressed in colorectal adenoma and cancer specimens and is negatively correlated with parameters of pathological aggressiveness and poor prognosis. (20824289)

References

- Distinguishing primary from secondary mucinous ovarian tumors: an algorithm using the novel marker DPEP1. Okamoto T, Matsumura N, Mandai M, Oura T, Yamanishi Y, Horiuchi A, Hamanishi J, Baba T, Koshiyama M, Shiozawa T, Konishi I. Mod Pathol. 2010 Nov 12. PMID: 21076463

- DPEP1, expressed in the early stages of colon carcinogenesis, affects cancer cell invasiveness. Toiyama Y, Inoue Y, Yasuda H, Saigusa S, Yokoe T, Okugawa Y, Tanaka K, Miki C, Kusunoki M. J Gastroenterol. 2010 Sep 8. PMID: 20824289

- Loss of expression of chromosome 16q genes DPEP1 and CTCF in lobular carcinoma in situ of the breast. Green AR, Krivinskas S, Young P, Rakha EA, Paish EC, Powe DG, Ellis IO. Breast Cancer Res Treat. 2009 Jan;113(1):59-66. PMID: 18213475

- Dipeptidase 1: a candidate tumor-specific molecular marker in colorectal carcinoma. McIver CM, Lloyd JM, Hewett PJ, Hardingham JE. Cancer Lett. 2004 Jun 8;209(1):67-74. PMID: 15145522