Thursday 22 April 2010
Definition: Parthenogenesis is a process used by some lizards and birds to reproduce without the need of a male.
For mammalians, the term is commonly referred to embryonic development of eggs activated artificially or aberrantly without fertilization by a sperm.
Although mammalian parthenocytes normally can not develop into a full organism, they could, in theory, provide a source to derive ES cells with an exact match to the oocyte donor’s genome (both nuclear and mitochondrial).
It is also possible that parthenogenesis could provide a source of cells that are homozygous for major histocompatibility alleles (such as HLA-A, B or DRB), thereby allowing partial MHC matching to a substantial population of unrelated transplant recipients.
It was found that recombination indeed occurs during meiosis I and II preceding parthenogenesis, and many alleles are not identical (or homozygous) in derived mouse parthenogenetic ES (PES) cells.
Nonetheless, establishing human PES cells (referred as hPES cells here) is an important milestone, not only for a potential source of human stem cells with improved histocompatibility, but also for future basic studies of epigenetic regulation and developmental/stem cell biology.
Genome-wide gene expression profiling reveals aberrant MAPK and Wnt signaling pathways associated with early parthenogenesis. Liu N, Enkemann SA, Liang P, Hersmus R, Zanazzi C, Huang J, Wu C, Chen Z, Looijenga LH, Keefe DL, Liu L. J Mol Cell Biol. 2010 Dec;2(6):333-44. PMID: #20926514# (Free)
More new lines of human parthenogenetic embryonic stem cells. Cheng L. Cell Res. 2008 Feb;18(2):215-7. PMID: #18246085#
Lin G, OuYang Q, Zhou X, et al. A highly homozygous and parthenogenetic human embryonic stem cell line derived from a one-pronuclear oocyte following in vitro fertilization procedure. Cell Res 2007; 17:999–1007.
Mai Q, Yu Y, Li T, et al. Derivation of human embryonic stem cell lines from parthenogenetic blastocysts. Cell Res 2007; 17:1008–1019. |
Revazova ES, Turovets NA, Kochetkova OD, et al. Patient-specific stem cell lines derived from human parthenogenetic blastocysts. Cloning Stem Cells 2007; 9:432–449.
Kim K, Ng K, Rugg-Gunn PJ, et al. Recombination signatures distinguish embryonic stem cells derived by parthenogenesis and somatic cell nuclear transfer. Cell Stem Cell 2007; 1:346–352.
Kim K, Lerou P, Yabuuchi A, et al. Histocompatible embryonic stem cells by parthenogenesis. Science 2007; 315:482–486.
Cheng L, Xiao L, Zeng F, Zhang YA. Stem Cells Shine in Shanghai. Cell Stem Cell 2008; 2:34–37.
Xu RH, Chen X, Li DS, et al. BMP4 initiates human embryonic stem cell differentiation to trophoblast. Nat Biotechnol 2002; 20:1261–1264.
Takahashi K, Tanabe K, Ohnuki M, et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 2007; 131:861–872.
Yu J, Vodyanik MA, Smuga-Otto K, et al. Induced pluripotent stem cell lines derived from human somatic cells. Science 2007; 318:1917–1920.