Sunday 7 March 2010
Transcriptomics studies often rely on partial reference transcriptomes that fail to capture the full catalogue of transcripts and their variations.
Recent advances in sequencing technologies and assembly algorithms have facilitated the reconstruction of the entire transcriptome by deep RNA sequencing (RNA-seq), even without a reference genome.
However, transcriptome assembly from billions of RNA-seq reads, which are often very short, poses a significant informatics challenge.
Next-generation transcriptome assembly. Martin JA, Wang Z. Nat Rev Genet. 2011 Sep 7;12(10):671-82. PMID: 21897427