Wednesday 29 October 2003
Splicing can be experimentally modified so that targeted exons are excluded from mature mRNA transcripts by blocking the access of splice-directing small nuclear ribonucleoprotein particles (snRNPs) to pre-mRNA using Morpholino antisense oligos. This has become a standard technique in developmental biology. Morpholino oligos can also be targeted to prevent molecules that regulate splicing (e.g. splice enhancers, splice suppressors) from binding to pre-mRNA, altering patterns of splicing.
Xing Y, Lee C. Alternative splicing and RNA selection pressure—evolutionary consequences for eukaryotic genomes. Nat Rev Genet. 2006 Jul;7(7):499-509. PMID: 16770337
Nissim-Rafinia M, Kerem B. The splicing machinery is a genetic modifier of disease severity. Trends Genet. 2005 Sep;21(9):480-3. PMID: 16039004
Barrass JD, Beggs JD. Splicing goes global. Trends Genet. 2003 Jun;19(6):295-8. PMID: 12801718
Cartegni L, Chew SL, Krainer AR. Listening to silence and understanding nonsense: exonic mutations that affect splicing. Nat Rev Genet. 2002 Apr;3(4):285-98. PMID: 11967553
Nissim-Rafinia M, Kerem B. Splicing regulation as a potential genetic modifier. Trends Genet. 2002 Mar;18(3):123-7. PMID: 11858835