Monday 20 October 2003
Definition: Proteomics is the study of the composition, structure, function and interactions of all the proteins in a cell.
Place the proteins
A central aim of cell biology is to find out where each protein functions in the cell.
In 2003, 75% of the yeast proteome was known. In 2003, two large-scale studies of Saccharomyces cerevisiae, involving labelling over 4,000 proteins with GFP fluorescence or TAP (tandem affinity purification) tags, have made it possible to determine which proteins are made and how many copies per cell, and to track down their subcellular locations, such as the nucleus, nucleolus, mitochondria and the Golgi apparatus. The database, and strain collection information are accessible at http://yeastgfp.ucsf.edu.
high-throughput protein arrays
Bader GD, Heilbut A, Andrews B, Tyers M, Hughes T, Boone C. Functional genomics and proteomics: charting a multidimensional map of the yeast cell. Trends Cell Biol. 2003 Jul;13(7):344-56. PMID: 12837605
Brunet S, Thibault P, Gagnon E, Kearney P, Bergeron JJ, Desjardins M. Organelle proteomics: looking at less to see more. Trends Cell Biol. 2003 Dec;13(12):629-38. PMID: 14624841
Patterson SD, Aebersold RH. Proteomics: the first decade and beyond. Nat Genet. 2003 Mar;33 Suppl:311-23. PMID: 12610541