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Home > Technical section > Biology > Molecular biology > DNA study > pyrosequencing


Sunday 4 February 2007

Pyrosequencing(R) is a unique system that allows the analysis of genetic variations including single-nucleotide polymorphisms, indels and short repeats, as well as assessing RNA allelic imbalance, DNA methylation status, and gene copy number.

Advances in methodology, including multiplex and universal primer applications, have reduced assay cost and improved throughput.


- Biotage


- Pyrosequencing of IDH1 and IDH2 Mutations in Brain Tumors and Non-neoplastic Conditions. Cykowski MD, Allen RA, Fung KM, Harmon MA, Dunn ST. Diagn Mol Pathol. 2012 Dec;21(4):214-20. PMID: 23111198

- Sensitive determination of BRAF copy number in clinical samples by pyrosequencing. Setty P, Gessi M, Waha A, Hammes J, El-Maarri O, Pietsch T, Waha A. Diagn Mol Pathol. 2011 Sep;20(3):148-57. PMID: 21817898

- Marsh S. Pyrosequencing(R) applications. Methods Mol Biol. 2006;373:15-24. PMID: 17185754

- Spittle C, Ward MR, Nathanson KL, Gimotty PA, Rappaport E, Brose MS, Medina A, Letrero R, Herlyn M, Edwards RH. Application of a BRAF pyrosequencing assay for mutation detection and copy number analysis in malignant melanoma. J Mol Diagn. 2007 Sep;9(4):464-71. PMID: 17690212