embryonic stem cells
Mammalian development requires the specification of over 200 cell types from a single totipotent cell.
Extracellular signals and second messengers modulate cell-autonomous regulators such as OCT4, SOX2 and Nanog in a combinatorial complexity.
Knowledge of this circuitry might reveal how to achieve phenotypic changes without the genetic manipulation of OCT4, Nanog and other toti/pluripotency-associated genes.
The differentiation of embryonic stem (ES) cells is accompanied by activation and/or silencing of specific loci and global chromatin reorganization. Hyperdynamic association between the chromatin and the major proteins that determine its architecture is a hallmark of pluripotency, and that it is required for differentiation to occur.
References
Green RM. Can we develop ethically universal embryonic stem-cell lines? Nat Rev Genet. 2007 Jun;8(6):480-5. PMID: 17510667
Nishikawa S, Jakt LM, Era T. Embryonic stem-cell culture as a tool for developmental cell biology. Nat Rev Mol Cell Biol. 2007 Jun;8(6):502-7. PMID: 17522593
Meshorer E, Misteli T. Chromatin in pluripotent embryonic stem cells and differentiation. Nat Rev Mol Cell Biol. 2006 Jul;7(7):540-6. PMID: 16723974
Solter D. From teratocarcinomas to embryonic stem cells and beyond: a history of embryonic stem cell research. Nat Rev Genet. 2006 Apr;7(4):319-27. PMID: 16534514
Boiani M, Scholer HR. Regulatory networks in embryo-derived pluripotent stem cells. Nat Rev Mol Cell Biol. 2005 Nov;6(11):872-84. PMID: 16227977
Keller G. Embryonic stem cell differentiation: emergence of a new era in biology and medicine. Genes Dev. 2005 May 15;19(10):1129-55. PMID: 15905405
Sperger JM, Chen X, Draper JS, Antosiewicz JE, Chon CH, Jones SB, Brooks JD, Andrews PW, Brown PO, Thomson JA. Gene expression patterns in human embryonic stem cells and human pluripotent germ cell tumors. Proc Natl Acad Sci U S A. 2003 Nov 11;100(23):13350-5. PMID: 14595015
Hochedlinger K, Jaenisch R. Nuclear transplantation, embryonic stem cells, and the potential for cell therapy.
N Engl J Med. 2003 Jul 17;349(3):275-86. PMID: 12867612
Rosenthal N. Prometheus’s vulture and the stem-cell promise. N Engl J Med. 2003 Jul 17;349(3):267-74. PMID: 12867611
Czyz J, Wobus A. Embryonic stem cell differentiation: the role of extracellular factors. Differentiation. 2001 Oct;68(4-5):167-74. PMID: 11776469
Hadjantonakis A, Papaioannou V. The stem cells of early embryos. Differentiation. 2001 Oct;68(4-5):159-66. PMID: 11776468